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Development of a refined tenocyte expansion culture technique for tendon tissue engineering
Author(s) -
Qiu Yiwei,
Wang Xiao,
Zhang Yaonan,
Carr Andrew J.,
Zhu Liwei,
Xia Zhidao,
Sabokbar Afsie
Publication year - 2014
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1597
Subject(s) - tendon , chemistry , tissue engineering , microbiology and biotechnology , fetal bovine serum , andrology , in vitro , immunology , anatomy , biomedical engineering , biology , medicine , biochemistry
The aim of this study was to efficiently expand less differentiated tenocytes with minimum use of fetal bovine serum (FBS) for tenocyte‐based tendon tissue engineering. To achieve this goal, human tenocytes were cultured in different concentrations of FBS and combinations of growth factors PDGF BB , IGF‐1 and bFGF. A number of growth factors were selected that could support tenocyte expansion at reduced differentiated state with minimum FBS usage. Results showed that the expansion of the tenocytes cultured for 14 days with 1% FBS, 50 ng/ml PDGF BB and 50 ng/ml bFGF was similar to that cultured in the 10% FBS control group. The tenocytes cultured in the treatment group showed significantly lower collagen synthesis and down‐regulation of mRNA expression of tendon differentiation markers. Cell morphology confirmed that tenocytes cultured in the growth factors had reduced collagen fibril formation compared to tenocytes cultured in 10% FBS. Our findings confirm the feasibility of inducing human tenocyte expansion in vitro with the least amount of FBS usage, while controlling their differentiation until required. Copyright © 2012 John Wiley & Sons, Ltd.