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In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds
Author(s) -
Satyam A.,
Subramanian G. S.,
Raghunath M.,
Pandit A.,
Zeugolis D. I.
Publication year - 2014
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1522
Subject(s) - genipin , ficoll , glutaraldehyde , chemistry , scaffold , fibroblast , tissue engineering , decellularization , biomedical engineering , biophysics , extracellular matrix , in vitro , biochemistry , chromatography , biology , chitosan , medicine , peripheral blood mononuclear cell
Polysaccharides are frequently incorporated into scaffolds for tissue engineering applications to improve mechanical and biological properties. We evaluated the influence of a Ficoll® scaffold on collagen films, a scaffold that is extensively used for soft and hard tissue repair. To avoid cytotoxicity issues associated with chemical reagents, the influence of genipin, a naturally occurring crosslinking agent, was assessed. Ultra‐structural level collagen films formed with and without Ficoll showed a fine fibrillar structure whereas genipin crosslinked films showed a coarse fibrillar and partially nodular structure. In contrast, glutaraldehyde crosslinked films lost their fibrillar pattern. Crosslinking significantly increased denaturation temperature ( p < 0.001), stress ( p < 0.0001) and force ( p < 0.0001) at break. Collagen/Ficoll and collagen/Ficoll/genipin films showed the highest WI38 fibroblast attachment than any other scaffold ( p < 0.003) and significantly greater WI38 fibroblast metabolic activity than other scaffolds ( p < 0.001). By day 6. collagen/Ficoll/genipin films also induced higher and more aligned fibronectin matrix deposition than other scaffolds. Overall, this study indicates the suitability of collagen/Ficoll/genipin for tissue engineering applications. Copyright © 2012 John Wiley & Sons, Ltd.