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Three‐dimensional growth matrix for human embryonic stem cell‐derived neuronal cells
Author(s) -
YläOutinen Laura,
Joki Tiina,
Varjola Mari,
Skottman Heli,
Narkilahti Susanna
Publication year - 2014
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1512
Subject(s) - embryonic stem cell , matrix (chemical analysis) , microbiology and biotechnology , neural stem cell , tissue engineering , neural tissue engineering , multielectrode array , neural cell , chemistry , stem cell , cell , biomedical engineering , neuroscience , anatomy , biology , nanotechnology , materials science , microelectrode , regeneration (biology) , engineering , biochemistry , electrode , chromatography , gene
The future of tissue engineering applications for neuronal cells will require a supportive 3D matrix. This particular matrix should be soft, elastic and supportive for cell growth. In this study, we characterized the suitability of a 3D synthetic hydrogel matrix, PuraMatrix™, as a growth platform for human embryonic stem cell (hESC)‐derived neural cells. The viability of the cells grown on top of, inside and under the hydrogel was monitored. The maturation and electrical activity of the neuronal networks inside the hydrogel were further characterized. We showed that cells stayed viable on the top of the PuraMatrix™ surface and growth of the neural cells and neural processes was good. Further, hESC‐derived neurons, astrocytes and oligodendrocytes all grew, matured and migrated when cultured inside the hydrogel. Importantly, neuronal cells were able to form electrically active connections that were verified using microelectrode array. Thus, PuraMatrix is a good supportive growth matrix for human neural cells and may serve as a matrix for neuronal scaffolds in neural tissue engineering. Copyright © 2012 John Wiley & Sons, Ltd.