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Efficient expansion of mesenchymal stem cells from mouse bone marrow under hypoxic conditions
Author(s) -
Yew TuLai,
Chang MingChau,
Hsu YuanTong,
He FanYu,
Weng WenHui,
Tsai ChihChien,
Chiu FangYao,
Hung ShihChieh
Publication year - 2013
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1491
Subject(s) - mesenchymal stem cell , bone marrow , microbiology and biotechnology , stem cell , biology , chemistry , immunology
Abstract To realize the therapeutic potential of mesenchymal stem cells (MSCs), a large number of high‐quality MSCs isolated from different species, such as mouse, were acquired for preclinical animal studies. Surprisingly, isolation and purification of mouse MSCs (mMSCs) is arduous because of the low frequency of MSCs and contamination of haematopoietic cells in culture. We have developed a method based on low density and hypoxic culture to isolate and expand mMSCs from different strains, including BALB/c, C57BL/6J, FVB/N and DBA/2. The cells from all of the strains expanded more rapidly when plated at low density in hypoxic culture compared with normoxic culture. These cells expressed CD44, CD105, CD29 and Sca‐1 markers but not CD11b, CD34, CD45 and CD31 markers. Moreover, they were able to differentiate along osteoblastic, adipocytic and chondrocytic lineages. In conclusion, we have developed a robust method for isolation and expansion of mMSCs by combining low‐density culture with hypoxic culture. Copyright © 2012 John Wiley & Sons, Ltd.