Premium
Ex vivo expansion of cord blood‐CD34 + cells using IGFBP 2 and Angptl‐5 impairs short‐term lymphoid repopulation in vivo
Author(s) -
Ventura Ferreira Mónica S.,
Labude Norina,
Walenda Gudrun,
Adamzyk Carina,
Wagner Wolfgang,
Piroth Daniela,
Müller Albrecht M.,
Knüchel Ruth,
Hieronymus Thomas,
Zenke Martin,
JahnenDechent Willi,
Neuss Sabine
Publication year - 2013
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1486
Subject(s) - haematopoiesis , cord blood , stem cell , cd34 , ex vivo , biology , transplantation , stem cell factor , immunology , repopulation , cytokine , andrology , in vivo , microbiology and biotechnology , medicine , genetics
Cord blood‐derived haematopoietic stem cells (CB‐HSCs) are an attractive source for transplantation in haematopoietic disorders. However, the yield of CB‐HSCs per graft is limited and often insufficient, particularly for the treatment of adult patients. Here we compare the capacity of three cytokine cocktails to expand CB‐CD34 + cells. Cells were cultured for 5 or 14 days in media supplemented with: (a) SCF, FL, IL‐3 and IL‐6 (SFLIL3/6); (b) SCF, TPO, FGF‐1 and IL‐6 (STFIL6); and (c) SCF, TPO, FGF‐1, IGFBP 2 and Angptl‐5 (STFAI). We observed that STFAI‐culture expansion sustained the most vigorous cell proliferation, maintenance of CD34 + phenotype and colony‐forming unit counts. In addition, STFAI‐cultured cells had a potent ex vivo migration activity. STFAI‐expanded cells were able to engraft NSG mice. However, no significant difference in overall engraftment was observed among the expansion cocktails. Assessment of short‐term reconstitution using multilineage markers demonstrated that the STFAI cocktail for HSCs expansion greatly improved total cell expansion but may impair short‐term lymphoid repopulation. Copyright © 2012 John Wiley & Sons, Ltd.