Premium
Development of a mechanically stable support for the osteoinductive biomaterial COLLOSS ® E
Author(s) -
Lode Anja,
Bernhardt Anne,
Kroonen Kathrin,
Springer Marco,
Briest Arne,
Gelinsky Michael
Publication year - 2009
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.138
Subject(s) - biomaterial , biomedical engineering , chemistry , alkaline phosphatase , composite number , stromal cell , gelatin , materials science , composite material , pathology , biochemistry , enzyme , medicine
The application of bone graft substitutes with osteoinductive properties is of high importance for the repair of large bone defects. COLLOSS ® E, a protein lyophilizate extracted from equine long bones, exhibits an osteoinductive potential which has been proven in several studies. In this work, a mechanically stable, but biodegradable support for COLLOSS ® E has been developed aiming at a bone graft substitute that retains shape and size when coming in contact with body fluids. Mineralization of collagen type I, isolated from horse tendon, resulted in a stable collagen hydroxyapatite nanocomposite. By means of freeze drying, this composite was used to prepare 3D scaffolds which can be filled with the cotton‐wool like COLLOSS ® E material. These scaffolds exhibit a porous microstructure and a good mechanical stability in dry and wet state. Cell culture experiments with human bone marrow stromal cells (hBMSC) revealed the cytocompatibility of the newly developed composite material. Cells were able to adhere, proliferate and differentiate into the osteoblastic lineage. The osteoinductive nature of COLLOSS ® E has been demonstrated by a significant higher activity of the osteogenic marker alkaline phosphatase (ALP) on combined scaffolds (mineralized collagen scaffolds filled with COLLOSS ® E) compared to pure scaffolds. The combination of COLLOSS ® E with scaffolds made of a collagen hydroxyapatite composite results in a synthetic bone graft substitute which can be completely remodelled into vital bone tissue opening an interesting new possibility for the therapy of bone defects. Copyright © 2008 John Wiley & Sons, Ltd.