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Solid emulsion gel as a vehicle for delivery of polyunsaturated fatty acids: implications for tissue repair, dermal angiogenesis and wound healing
Author(s) -
Shingel Kirill I.,
Faure MariePierre,
Azoulay Laurent,
Roberge Christophe,
Deckelbaum Richard J.
Publication year - 2008
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.101
Subject(s) - angiogenesis , wound healing , chemistry , microbiology and biotechnology , vascular endothelial growth factor , in vivo , biochemistry , biology , cancer research , immunology , vegf receptors
The paper describes preparation and biological characterization of the solid hybrid biomaterial that was designed for cell‐targeted lipid delivery in healing tissues. The material referred to as ‘solid emulsion gel’ combines a protein‐stabilized lipid emulsion and a hydrogel structure in a single compartment. The potential of the omega‐3 (n‐3)‐fatty acids rich solid emulsion gel for tissue repair applications was investigated at the macro‐, micro‐, molecular and gene expression levels, using human fibroblasts and endothelial cells and a porcine model of full‐thickness wounds. Being non‐cytotoxic in vitro and in vivo , the biomaterial was found to affect cell metabolism, modulate expression of certain genes, stimulate early angiogenesis and promote wound repair in vivo . The neovascular response in vivo was correlated with upregulated expression of the genes involved in lipid transport (e.g. adipophilin), anti‐apoptosis (e.g. heat shock proteins, haem oxygenase 1) and angiogenesis (vascular endothelial growth factor, placental growth factor). Collectively, the results of this study provide first evidence that the angiogenic response provided by solid emulsion gel‐mediated delivery of n‐3 fatty acids is an alternative to the topical administration of exogenous growth factors or gene therapy, and can be advantageously used for the stimulation of tissue repair in complex wounds. Copyright © 2008 John Wiley & Sons, Ltd.