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Inactivation of Escherichia coli in Ice through DC electric field application and freezing
Author(s) -
Murakami Yuichi,
Sato Takunao,
Goto Yuma,
Muramoto Yuji
Publication year - 2021
Publication title -
ieej transactions on electrical and electronic engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.254
H-Index - 30
eISSN - 1931-4981
pISSN - 1931-4973
DOI - 10.1002/tee.23323
Subject(s) - electric field , sterilization (economics) , analytical chemistry (journal) , electrode , cuvette , aqueous solution , escherichia coli , chemistry , voltage , materials science , chromatography , electrical engineering , physics , biochemistry , optics , engineering , quantum mechanics , monetary economics , economics , foreign exchange market , gene , foreign exchange
This study presents the effect of direct current (DC) electric field application on ice sterilization. The sample used was Escherichia coli (JM103) in a 0.001, 0.01, or 0.1 mol/L NaCl aqueous solution. The experimental sample was placed in a cuvette with parallel electrode plates (distance between electrodes: 1 mm) and then cooled at −30 °C or −80 °C for 40 min. After freezing, a 100 V DC voltage was applied to the sample in the freezer with a temperature of −30 °C or −80 °C. Consequently, the survival ratio of E. coli in all samples after freezing was lower than that before freezing due to the freezing injury. In conclusion, we found the bactericidal effect of the DC electric fields in the frozen 0.01 mol/L NaCl samples. © 2021 Institute of Electrical Engineers of Japan. Published by Wiley Periodicals LLC.