Premium
Effects of extremely low frequency magnetic fields on neuronal development of P19 embryonal carcinoma cells
Author(s) -
Saito Atsushi,
Takayama Yuzo,
Moriguchi Hiroyuki,
Kotani Kiyoshi,
Jimbo Yasuhiko
Publication year - 2011
Publication title -
ieej transactions on electrical and electronic engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.254
H-Index - 30
eISSN - 1931-4981
pISSN - 1931-4973
DOI - 10.1002/tee.20638
Subject(s) - p19 cell , neurite , embryonal carcinoma , embryoid body , biology , induced pluripotent stem cell , cellular differentiation , extracellular , neuroscience , stimulation , stem cell , embryonic stem cell , microbiology and biotechnology , pathology , chemistry , medicine , in vitro , biochemistry , gene
Abstract Modulation of pluripotent stem cell differentiation by several environmental factors, such as physical stimulation, is an important theme in tissue engineering. In this study, we report the effects of extremely low frequency magnetic field (ELF‐MF) exposure on the neuronal differentiation ability of P19 embryonal carcinoma cells (P19 cells). Here, the embryoid bodies (EBs) formed during the induction process were exposed to two types of ELF‐MFs (1 or 10 mT, 50 Hz, sinusoidal). After the induction process, the effects on the development of neuronal cells were evaluated by phase‐contrast image analysis, immunohistochemical staining (MAP2, GFAP), and the extracellular recording of spontaneous activity in which microelectrode arrays (MEAs) were used. Results show that MAP2 positive cells and spike frequencies increased by 10 mT ELF‐MF exposure, but the percentage of neurite outgrowth decreased at the early stages of differentiation. In contrast, clear differences were not seen in 1 mT ELF‐MF exposed cells. These results suggest that the neuronal differentiation and network properties were affected by 10 mT ELF‐MF exposure and that the intensity of the magnetic field was one of the important factors in the neuronal development of P19 cells. © 2011 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.