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Portable imaging system for on‐site analysis using CMOS imager—microfluidic analysis and fluorescence imaging
Author(s) -
Misawa Nobuo,
Takeuchi Shoji
Publication year - 2011
Publication title -
ieej transactions on electrical and electronic engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.254
H-Index - 30
eISSN - 1931-4981
pISSN - 1931-4973
DOI - 10.1002/tee.20630
Subject(s) - oled , microfluidics , cmos , materials science , fluorescence , optoelectronics , fluorescence lifetime imaging microscopy , nanotechnology , optics , physics , layer (electronics)
This paper reports on a portable imaging system using a complementary metal oxide semiconductor (CMOS) imager for microfluidic analysis and fluorescence imaging with an organic light emitting diode (OLED). The portable CMOS imager is useful for onsite microfluidic analysis, and the OLED works as a light source for fluorescent observations. The system needs only five elements: a laptop computer, a CMOS imaging device, an optical filter, microfluidic channels, and OLEDs. Using this system, we succeeded in a bead‐based microfluidic assay, capturing the video of the trapping and releasing process. Furthermore, we observed the fluorescent beads and the dye using an OLED as the light source in this system. As a result, we found that (i) submillimeter objects could be in focus with about 1 mm distance from CMOS imaging device surface without a lens; (ii) microfluidic experiments with poly(dimethylsiloxane) channels were possible on the CMOS imager; and (iii) OLED can potentially be used as a light source for this system. We believe that this system is useful for point‐of‐care diagnostic sensing and onsite microfluidic analysis including fluorescence detection. © 2011 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.