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Reactivity and Selectivity Principles in Native Protein Bioconjugation
Author(s) -
Adakkattil Ramesh,
Thakur Kalyani,
Rai Vishal
Publication year - 2021
Publication title -
the chemical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.61
H-Index - 78
eISSN - 1528-0691
pISSN - 1527-8999
DOI - 10.1002/tcr.202100108
Subject(s) - bioconjugation , chemoselectivity , combinatorial chemistry , modularity (biology) , toolbox , chemistry , modular design , computational biology , computer science , nanotechnology , biochemistry , biology , materials science , programming language , genetics , catalysis
Are chemical methods capable of precisely engineering the native proteins? Is it possible to develop platforms that can empower the regulation of chemoselectivity, site‐selectivity, modularity, protein‐specificity, and site‐specificity? This account delineates our research journey in the last ten years on the developments revolving around these questions. It will range from the realization of chemoselective and site‐selective labeling of reactivity hotspots to modular linchpin directed modification (LDM®) platform and site‐specific Gly‐tag® technology. Also, we outline a few biotechnology tools, including Maspecter®, that accelerated the detailed analysis of the bioconjugates and rendered a powerful toolbox for homogeneous antibody‐drug conjugates (ADCs).