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Luminescent Strategies for Label‐Free G‐Quadruplex‐Based Enzyme Activity Sensing
Author(s) -
Ma DikLung,
Dong ZhenZhen,
Vellaisamy Kasipandi,
Cheung KaMan,
Yang Guanjun,
Leung ChungHang
Publication year - 2017
Publication title -
the chemical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.61
H-Index - 78
eISSN - 1528-0691
pISSN - 1527-8999
DOI - 10.1002/tcr.201700014
Subject(s) - enzyme , g quadruplex , luminescent measurements , computational biology , biochemistry , chemistry , luminescence , biology , combinatorial chemistry , dna , materials science , optoelectronics
By catalyzing highly specific and tightly controlled chemical reactions, enzymes are essential to maintaining normal cellular physiology. However, aberrant enzymatic activity can be linked to the pathogenesis of various diseases. Therefore, the unusual activity of particular enzymes can represent testable biomarkers for the diagnosis or screening of certain diseases. In recent years, G‐quadruplex‐based platforms have attracted wide attention for the monitoring of enzymatic activities. In this Personal Account, we discuss our group's works on the development of G‐quadruplex‐based sensing system for enzyme activities by using mainly iridium(III) complexes as luminescent label‐free probes. These studies showcase the versatility of the G‐quadruplex for developing assays for a variety of different enzymes.