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Development and application of high‐performance affinity beads: Toward chemical biology and drug discovery
Author(s) -
Sakamoto Satoshi,
Kabe Yasuaki,
Hatakeyama Mamoru,
Yamaguchi Yuki,
Handa Hiroshi
Publication year - 2009
Publication title -
the chemical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.61
H-Index - 78
eISSN - 1528-0691
pISSN - 1527-8999
DOI - 10.1002/tcr.20170
Subject(s) - affinity chromatography , drug discovery , drug , drug development , computational biology , biochemical engineering , chemistry , combinatorial chemistry , biology , biochemistry , pharmacology , enzyme , engineering
In drug development research, the elucidation and understanding of the interactions between physiologically active substances and proteins that numerous genes produce is important. Currently, most commercially available drugs and physiologically active substances have been brought to market without knowledge of factors interacting with the drugs and the substances. Affinity purification is a useful and powerful technique employed to understand factors that are targeted by drugs and physiologically active substances. However, use of conventional matrices for affinity chromatography often causes a decrease in efficiency of affinity purification and, as a result, more practical matrices for affinity purification have been developed for application in drug discovery research. In this paper, we describe the development of high‐performance affinity beads (SG beads and FG beads) that enable one‐step affinity purification of drug targets and the elucidation of the mechanism of the action of the drugs. We also describe a chemical screening system using our affinity beads. We hope that utilization of the affinity beads will contribute to the progress of research in chemical biology. © 2009 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Chem Rec 9: 66–85; 2009: Published online in Wiley InterScience ( www.interscience.wiley.com ) DOI 10.1002/tcr.20170