Premium
Single‐molecule measurements with a single quantum dot
Author(s) -
Kaji Noritada,
Tokeshi Manabu,
Baba Yoshinobu
Publication year - 2007
Publication title -
the chemical record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.61
H-Index - 78
eISSN - 1528-0691
pISSN - 1527-8999
DOI - 10.1002/tcr.20128
Subject(s) - quantum dot , cadmium selenide , photobleaching , cadmium telluride photovoltaics , molecule , nanotechnology , fluorophore , chemistry , in vivo , materials science , fluorescence , physics , optics , biology , microbiology and biotechnology , organic chemistry
Recent progress of quantum dot (QD) applications in single‐molecule measurements are reviewed in this paper. Bright fluorescence and anti‐photobleaching properties of QDs have explored the way to conduct long‐time trajectory tracking of transmembrane proteins both in vitro and in vivo . Coupled with diversities of chemical and biochemical modifications of QD surfaces, their application fields are expanding to multidiscipline fields including imaging on the basis of a single molecule. Currently, molecular interactions and conformational changes on the QD surface can be detected at a single‐molecule level. These expansions of application fields also involve toxicity problems in cells since most commercially available QDs consist of cadmium selenide or cadmium telluride, which are inherently toxic. For widespread applications of QDs including in vivo and therapeutic use in place of current organic fluorophore, cytotoxicity is discussed as well in this paper. © 2007 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Chem Rec 7: 295–304; 2007: Published online in Wiley InterScience ( www.interscience.wiley.com )DOI 10.1002/tcr.20128