Open AccessCompact portable multiphoton microscopy reveals histopathological hallmarks of unprocessed lung tumor tissue in real time
Author(s) -
Huizen Laura M. G.,
Radonic Teodora,
Mourik Frank,
Seinstra Danielle,
Dickhoff Chris,
Daniels Johannes M. A.,
Bahce Idris,
Annema Jouke T.,
Groot Marie Louise
Publication year - 2020
Publication title -
translational biophotonics
Language(s) - English
Resource type - Journals
ISSN - 2627-1850
DOI - 10.1002/tbio.202000009
Subject(s) - pathology , autofluorescence , elastin , microscopy , lung cancer , lung , adenocarcinoma , multiphoton fluorescence microscope , histology , materials science , biomedical engineering , medicine , fluorescence microscope , cancer , optics , fluorescence , physics
During lung cancer operations a rapid and reliable assessment of tumor tissue can reduce operation time and potentially improve patient outcomes. We show that third harmonic generation (THG), second harmonic generation (SHG) and two‐photon excited autofluorescence (2PEF) microscopy reveals relevant, histopathological information within seconds in fresh unprocessed human lung samples. We used a compact, portable microscope and recorded images within 1 to 3 seconds using a power of 5 mW. The generated THG/SHG/2PEF images of tumorous and nontumorous tissues are compared with the corresponding standard histology images, to identify alveolar structures and histopathological hallmarks. Cellular structures (tumor cells, macrophages and lymphocytes) (THG), collagen (SHG) and elastin (2PEF) are differentiated and allowed for rapid identification of carcinoid with solid growth pattern, minimally enlarged monomorphic cell nuclei with salt‐and‐pepper chromatin pattern, and adenocarcinoma with lipidic and micropapillary growth patterns. THG/SHG/2PEF imaging is thus a promising tool for clinical intraoperative assessment of lung tumor tissue.