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A proposal for a standardised protocol to barcode all land plants
Author(s) -
Chase Mark W.,
Cowan Robyn S.,
Hollingsworth Peter M.,
van den Berg Cassio,
Madriñán Santiago,
Petersen Gitte,
Seberg Ole,
Jørgsensen Tina,
Cameron Kenneth M.,
Carine Mark,
Pedersen Niklas,
Hedderson Terry A.J.,
Conrad Ferozah,
Salazar Gerardo A.,
Richardson James E.,
Hollingsworth Michelle L.,
Barraclough Timothy G.,
Kelly Laura,
Wilkinson Mike
Publication year - 2007
Publication title -
taxon
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 81
eISSN - 1996-8175
pISSN - 0040-0262
DOI - 10.1002/tax.562004
Subject(s) - rpob , dna barcoding , plastid , barcode , mitochondrial dna , biology , coding (social sciences) , computational biology , protocol (science) , evolutionary biology , computer science , genetics , gene , chloroplast , mathematics , statistics , medicine , alternative medicine , pathology , operating system , 16s ribosomal rna
Abstract We propose in this paper to use three regions of plastid DNA as a standard protocol for barcoding all land plants. We review the other markers that have been proposed and discuss their advantages and disadvantages. The low levels of variation in plastid DNA make three regions necessary; there are no plastid regions, coding or non‐coding, that evolve as rapidly as mitochondrial DNA generally does in animals. We outline two, three‐region options, (1) rpoC1, rpoB and 1matK or (2) rpoC1, matK and psbA‐trnH as viable markers for land plant barcoding.