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Cell‐Free Protein Synthesis in Bifunctional Hyaluronan Microgels: A Strategy for In Situ Immobilization and Purification of His‐Tagged Proteins
Author(s) -
Heida Thomas,
Köhler Tony,
Kaufmann Anika,
Männel Max J.,
Thiele Julian
Publication year - 2020
Publication title -
chemsystemschem
Language(s) - English
Resource type - Journals
ISSN - 2570-4206
DOI - 10.1002/syst.202000020
Subject(s) - bifunctional , chemistry , front cover , in situ , matrix (chemical analysis) , polymer science , biophysics , polymer chemistry , chemical engineering , cover (algebra) , biochemistry , chromatography , biology , organic chemistry , engineering , mechanical engineering , catalysis
The front cover artwork is provided by the Thiele group at Leibniz IPF Dresden (Germany). The image shows a microgel hosting cell‐free synthesis of green fluorescent protein. Despite its open, membrane‐free nature, the multifunctional microgel is also able to trap proteins inside its polymer matrix. Read the full text of the Article at 10.1002/syst.201900058.