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Expression of adenosine A 1 receptors in cultured neurons from fetal rat brain
Author(s) -
Nicolas Frédéric,
Daval JeanLuc
Publication year - 1993
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.890140113
Subject(s) - ccpa , receptor , adenosine , adenosine receptor , adenosine a1 receptor , forebrain , chemistry , population , adenosine a2b receptor , medicine , gtp' , endocrinology , microbiology and biotechnology , biology , biochemistry , agonist , central nervous system , enzyme , environmental health
The expression of adenosine A 1 receptors was investigated using [ 3 H]2‐chloro‐N 6 ‐cyclopentyladenosine (CCPA) in 8‐day‐old cultured neurons from fetal rat forebrain grown in serum‐free medium. [ 3 H]CCPA bound specifically and with high affinity (Kd = 2.9 nM) to a homogeneous population of sites. Displacement of CCPA binding by various adenosine derivatives indicated that A 1 receptors were selectively labeled. The presence of Gpp(NH)p, a GTP analogue, reduced significantly the binding affinity (Kd = 12.2 nM), suggesting that A 1 receptors detected in intact cultured cells are linked to associated G proteins. © 1993 Wiley‐Liss, Inc.
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