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Regional distribution of neuropeptide somatostatin gene expression in the human brain
Author(s) -
Mengod G.,
Rigo M.,
Savasta M.,
Probst A.,
Palacios J. M.
Publication year - 1992
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.890120108
Subject(s) - globus pallidus , in situ hybridization , zona incerta , nucleus accumbens , substantia nigra , neuropeptide , thalamus , human brain , basal ganglia , caudate nucleus , putamen , biology , somatostatin , nucleus , cerebellum , medulla oblongata , neocortex , neuroscience , central nervous system , gene expression , gene , dopaminergic , dopamine , biochemistry , receptor
The regional distribution of mRNA coding for the neuropeptide somatostatin has been studied in the human brain by in situ hybridization histochemistry using 32 P‐labeled oligonucleotides. We show that somatostatin mRNA‐containing neurons are widely distributed in a number of nuclei and grey areas of the human brain, including neocortex, putamen, nucleus caudatus, nucleus accumbens, amygdala, midbrain, medulla oblongata, hippocampal formation, reticular nucleus of the thalamus, and posterior nucleus of the hypothalamus. No significant hybridization signal was observed in the substantia nigra, claustrum, globus pallidus, thalamus, and cerebellum. The topographic localization of neurons containing SOM mRNA in the human brain is in agreement with previous studies using immunocytochemical or radioimmunoassay techniques. These results show that in situ hybridization histochemistry with oligonucleotide probes can be used to map the distribution of neurons expressing SOM mRNA in human postmortem materials. © 1992 Wiley‐Liss, Inc.