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Arylamine spider toxins antagonize NMDA receptor‐mediated synaptic transmission in rat hippocampal slices
Author(s) -
Mueller Alan L.,
Albensi Benedict C.,
Gag Alan H.,
Reynolds Linda S.,
Jackson Hunter
Publication year - 1991
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.890090403
Subject(s) - dnqx , ampa receptor , nmda receptor , spider toxin , population , chemistry , neurotransmission , dizocilpine , hippocampal formation , pharmacology , population spike , kainate receptor , photostimulation , biology , excitatory postsynaptic potential , receptor , glutamate receptor , neuroscience , biochemistry , medicine , environmental health
The effects of arylamine spider toxins on synaptic transmission in rat hippocampal slices were investigated. Two different responses were monitored: the AMPA receptor‐mediated population spike recorded in control buffer (selectively antagonized by DNQX) and the NMDA receptor‐mediated EPSP recorded in nominally magnesium‐free buffer containing 20 μM DNQX (selectively antagonized by AP5, AP7, and dizocilpine (MK‐801)). The synthetic arylamine spider toxins JSTX‐3, argiotoxin‐636, and argiotoxin‐659 were 26 to 73 times more potent at antagonizing the NMDA receptor‐mediated EPSP (IC 50 values ranging from 12 to 24 μM) than the AMPA receptor‐mediated population spike (IC 50 values ranging from 612 to 878 μM). These results indicate that arylamine spider toxins are selective antagonists of NMDA receptors in the mammalian CNS.