Premium
Pharmacology of N‐methyl‐D‐aspartate‐induced brain injury in an in vivo perinatal rat model
Author(s) -
McDonald John. W.,
Johnston Michael V.
Publication year - 1990
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.890060210
Subject(s) - nmda receptor , neuroprotection , pharmacology , in vivo , glutamate receptor , excitotoxicity , chemistry , medicine , anesthesia , receptor , biology , biochemistry , microbiology and biotechnology
Abstract Intrastratal injection of the glutamate analogue N‐methyl‐D‐aspartate (NMDA, 25 nmol) in postnatal (PND) 7 rats provides a rapid, sensitive, and reproducible assay in which potential neuroprotective strategies against excitotoxic neuronal injury can be examined in vivo. Brain injury is quantified 5 days postinjection by comparison of the weights of the injected and contralateral cerebral hemispheres. Intraperitoneal injections (15 minutes post‐NMDA) of competitive and noncometitive NMDA receptor antagonists attenuated the severity of NMDA‐induced brain injury. The rank order of neuroprotective potency of these antagonists was CGS‐19755