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Neurotensin binding sites in porcine jejunum: Biochemical Characterization and intramural Localization
Author(s) -
Seybold Virginia S.,
Treder Bradley G.,
Aaonsen Linda M.,
Parsons Ann,
Brown David R.
Publication year - 1990
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.890060110
Subject(s) - neurotensin , jejunum , submucosa , myenteric plexus , medicine , chemistry , dissociation constant , endocrinology , ileum , biology , anatomy , neuropeptide , receptor , biochemistry , immunohistochemistry
Neurotensin is present in high concentrations in the mammalian gut, especially in enteroendocrine cells of the mucose. Exogenous neurotensin has been shown to alter ion transport by the mucosa and contractile activity of intestinal smooth muscle. The purpose of this study was to determine the distribution of neurotensin binding sites withing the intestinal wall. Initially, biochemical characteristics of [ 125 I]neurotensin binding sites were determined within two preparations of the distal porcine jejunum: (1) the mucosa and submucosa, and (2) the circular and longitudinal muscle with their intramural plexuses. Ligand binding data for the preparation including the mucosa and submucosa indicated that [ 125 I]neurotensin bound specifically to two sites having apparent equilibrium dissociation constants of approximately 0.46 and 0.37nM. A binding site with a dissociation constant of approximately 0.38 nM was confirmed for the preparation of muscle and associated intramural plexuses. Xenopsin and neurotensin