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Investigation of behavioral and electrophysiological responses induced by selective stimulation of CCKB receptors by using a new highly potent CCK analog, BC 264
Author(s) -
Dauge V.,
Bohme G. A.,
Crawley J. N.,
Durieux C.,
Stutzmann J. M.,
Feger J.,
Blanchard J. C.,
Roques B. P.
Publication year - 1990
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.890060109
Subject(s) - cholecystokinin , nucleus accumbens , chemistry , long term potentiation , stimulation , receptor , dopamine , cholecystokinin b receptor , cholecystokinin receptor , medicine , hippocampal formation , endocrinology , dopamine receptor , neuroscience , pharmacology , biology , biochemistry
The new CCK B analog, Boc‐Tyr (SO 3 H)‐gNle‐mGly‐Trp‐(NMe)‐Nle‐Asp‐PheNH 2 (BC 264) exhibited a high affinity (KI = 0.39 ± 0.15 nM) and selectivity for central (B) versus peripheral (A) receptors (KI CCK A /KI CCK B = 910) in the rat. In agreement with these binding studies, BC 264 was at least 50 times more potent than CCK 8 in stimulating the firing of rat CA hippocampal neurones. Furthermore sterotzxic injection of BC 264 or CCK 8 in the VTA of rats resulted in potentiation of the dopamine‐induced hypolocomotion. These two types of CCK 8 responses have been previously shown to involve CCK B receptrs. In contrast, after administration into the posteor‐median nucleus accumbens, the hypoexploration, the increase of emotionality of rats, or the potentiation of dopamine‐induced hyperlocomotion were obtained after injection of CCK 8 but not of BC 264, supporting the involvement of peripheral CCK A receptors in these CCK 8 responses. Owing to its resistance to peptidases, BC 264 appears to be of great interest in the investigation of the still uncertain funcitonal roles of CCK in the central nervous system.