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Anatomical localization of calmodulin mRNA in the rat brain with cloned cDNA and synthetic oligonucleotide probes
Author(s) -
RobertsLewis Jill M.,
Cimino Mauro,
Krause Rudolph G.,
Tyrrell Daniel F.,
Davis Leonard G.,
Weiss Benjamin,
Lewis Michael E.
Publication year - 1990
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.890050311
Subject(s) - calmodulin , complementary dna , oligonucleotide , messenger rna , in situ hybridization , biology , hippocampus , striatum , microbiology and biotechnology , biochemistry , enzyme , neuroscience , gene , dopamine
Calmodulin is a small, acidic calcium‐binding protein that regulates a number of calcium‐dependent enzyme activities and is thought to be involved in neurotransmission to begin to explore further the regulation of this important protein in the brain, we have cloned a rat calmodulin cDNA and designed an oligonucleotide probe based on this sequence. Both the cDNA and oligonucleotide probes revealed a markedly heterogeneous distribution of hybridization signal for calmodulin mRNA in the rat brain. The greatest apparent abundance of mRNA for calmodulin was seen in the hippocampus and cerebral cortex, whereas many brain regions showed relatively low hybridization signal, including the striatum and portions of the hypothalamus and brainstem.