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Evidence for the co‐storage and co‐release of neuropeptide y and noradrenaline from large dense cored vesicles in sympathetic nerves of the bovine vas deferens
Author(s) -
de Potter W. P.,
Dillen L.,
Annaert W.,
Tombeur K.,
Berghmans R.,
Coen E. P.
Publication year - 1988
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.890020207
Subject(s) - vas deferens , neuropeptide y receptor , neuropeptide , vesicle , medicine , endocrinology , norepinephrine , differential centrifugation , chemistry , free nerve ending , adrenergic , biology , dopamine , biochemistry , receptor , membrane
Abstract The subcellular localization and the secretion of neuropeptide Y were studied in sympathetic nerve endings of bovine vas deferens. Immunostaining revealed a parallel distribution for neuropeptide Y and dopamine‐β‐hydroxylase immunoreactivity in the network of varicose nerve fibers in the smooth muscle layers of the vas deferens. Following differential centrifugation and sucrose density gradient centrifugation, neuropeptide Y was found to coincide with noradrenaline in the more dense region of the gradient, where the large dense cored vesicles are found. Superfusion experiments demonstrated the release of neuropeptide Y and noradrenaline upon electrical stimulation. Furthermore, the neuropeptide Y secretion was shown to be Ca 2+ ‐dependent. We conclude that in the bovine vas deferens neuropeptide Y is only present in large dense cored vesicles of adrenergic neurons and that the peptide and noradrenaline are coreleased from these vesicles in a calcium dependent manner.