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Monitoring of glutamate‐induced excitotoxicity by mitochondrial oxygen consumption
Author(s) -
Kumagai Ayako,
Sasaki Tsutomu,
Matsuoka Kenta,
Abe Masayoshi,
Tabata Toshihide,
Itoh Yumi,
Fuchino Hiroyuki,
Wugangerile Sartagul,
Suga Mika,
Yamaguchi Tomoko,
Kawahara Hidehisa,
Nagaoka Yasuo,
Kawabata Kenji,
Furue Miho Kusuda,
Takemori Hiroshi
Publication year - 2019
Publication title -
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Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.22067
Subject(s) - excitotoxicity , glutamate receptor , neurotoxicity , mitochondrion , microbiology and biotechnology , membrane potential , intracellular , astrocyte , biology , biochemistry , chemistry , biophysics , neuroscience , receptor , toxicity , central nervous system , organic chemistry
Abstract Dysfunction of mitochondrial activity is often associated with the onset and progress of neurodegenerative diseases. Membrane depolarization induced by Na + influx increases intracellular Ca 2+ levels in neurons, which upregulates mitochondrial activity. However, overlimit of Na + influx and its prolonged retention ultimately cause excitotoxicity leading to neuronal cell death. To return the membrane potential to the normal level, Na + /K + ‐ATPase exchanges intracellular Na + with extracellular K + by consuming a large amount of ATP. This is a reason why mitochondria are important for maintaining neurons. In addition, astrocytes are thought to be important for supporting neighboring neurons by acting as energy providers and eliminators of excessive neurotransmitters. In this study, we examined the meaning of changes in the mitochondrial oxygen consumption rate (OCR) in primary mouse neuronal populations. By varying the medium constituents and using channel modulators, we found that pyruvate rather than lactate supported OCR levels and conferred on neurons resistance to glutamate‐mediated excitotoxicity. Under a pyruvate‐restricted condition, our OCR monitoring could detect excitotoxicity induced by glutamate at only 10 μM. The OCR monitoring also revealed the contribution of the N‐methyl‐D‐aspartate receptor and Na + /K + ‐ATPase to the toxicity, which allowed evaluating spontaneous excitation. In addition, the OCR monitoring showed that astrocytes preferentially used glutamate, not glutamine, for a substrate of the tricarboxylic acid cycle. This mechanism may be coupled with astrocyte‐dependent protection of neurons from glutamate‐mediated excitotoxicity. These results suggest that OCR monitoring would provide a new powerful tool to analyze the mechanisms underlying neurotoxicity and protection against it.