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Changes in the expression of IL‐6‐Mediated MicroRNAs in the dorsal root ganglion under neuropathic pain in mice
Author(s) -
Hori Naosuke,
Narita Michiko,
Yamashita Akira,
Horiuchi Hiroshi,
Hamada Yusuke,
Kondo Takashige,
Watanabe Moe,
Igarashi Katsuhide,
Kawata Miho,
Shibasaki Masahiro,
Yamazaki Mitsuaki,
Kuzumaki Naoko,
Inada Eiichi,
Ochiya Takahiro,
Iseki Masako,
Mori Tomohisa,
Narita Minoru
Publication year - 2016
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.21902
Subject(s) - dorsal root ganglion , neuropathic pain , sciatic nerve , ligation , microrna , knockout mouse , medicine , spinal cord , anesthesia , biology , neuroscience , receptor , gene , biochemistry
A multiplex analysis for profiling the expression of candidate microRNAs (miRNAs), which are small noncoding RNAs that function as key post‐transcriptional regulators, may lead to a better understanding of the complex machinery of neuropathic pain. In the present study, we performed a miRNA array analysis using tissues of the dorsal root ganglion (DRG), a primary site for pain processing, obtained from mice with partial sciatic nerve ligation. Among 1135 total miRNAs, 26 miRNAs showed up‐regulation (more than 2‐fold change) and only 4 miRNAs showed down‐regulation (less than 0.5‐fold change) in the DRG of nerve‐ligated mice. In a RT‐qPCR assay, the levels of miR‐21, miR‐431, and miR‐511‐3p were significantly increased on the ipsilateral side of the DRG from 3 to 7 days after sciatic nerve ligation. These elevations were almost absent in IL‐6 knockout mice. Furthermore, the expression level of miR‐21, but not those of miR‐431 or miR511‐3p, was significantly increased in exosomes extracted from blood of nerve‐ligated mice. These findings suggest that the increased expression of IL‐6‐regulated miR‐21, miR‐431, and miR‐511‐3p in the DRG and increased exosomal miR‐21 extracted from blood after sciatic nerve ligation may play at least a partial role in neuropathic pain. Synapse 70:317–324, 2016 . © 2016 Wiley Periodicals, Inc.