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G‐protein‐coupled inward rectifier potassium channels involved in corticostriatal presynaptic modulation
Author(s) -
Meneses David,
Mateos Verónica,
Islas Gustavo,
Barral Jaime
Publication year - 2015
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.21833
Subject(s) - neurotransmission , inward rectifier potassium ion channel , neuroscience , chemistry , potassium channel , striatum , neurotransmitter , sk channel , biophysics , receptor , ion channel , biology , dopamine , central nervous system , biochemistry
Presynaptic modulation has been associated mainly with calcium channels but recent data suggests that inward rectifier potassium channels ( K IR ) also play a role. In this work we set to characterize the role of presynaptic K IR channels in corticostriatal synaptic transmission. We elicited synaptic potentials in striatum by stimulating cortical areas and then determined the synaptic responses of corticostriatal synapsis by using paired pulse ratio (PPR) in the presence and absence of several potassium channel blockers. Unspecific potassium channels blockers Ba 2+ and Cs + reduced the PPR, suggesting that these channels are presynaptically located. Further pharmacological characterization showed that application of tertiapin‐Q, a specific K IR 3 channel family blocker, also induced a reduction of PPR, suggesting that K IR 3 channels are present at corticostriatal terminals. In contrast, exposure to Lq2, a specific K IR 1.1 inward rectifier potassium channel, did not induce any change in PPR suggesting the absence of these channels in the presynaptic corticostriatal terminals. Our results indicate that K IR 3 channels are functionally expressed at the corticostriatal synapses, since blockage of these channels result in PPR decrease. Our results also help to explain how synaptic activity may become sensitive to extracellular signals mediated by G‐protein coupled receptors. A vast repertoire of receptors may influence neurotransmitter release in an indirect manner through regulation of K IR 3 channels. Synapse 69:446–452, 2015 . 2015 Wiley Periodicals, Inc.