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Hippocampal serotonin‐1A receptor function in a mouse model of anxiety induced by long‐term voluntary wheel running
Author(s) -
Fuss Johannes,
Vogt Miriam A.,
Weber KlausJosef,
Burke Teresa F.,
Gass Peter,
Hensler Julie G.
Publication year - 2013
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.21666
Subject(s) - hippocampal formation , dentate gyrus , anxiogenic , receptor , neuroscience , hippocampus , chemistry , 5 ht1a receptor , endocrinology , medicine , psychology , 5 ht receptor , serotonin , biology , anxiolytic , biochemistry
We have recently demonstrated that, in C57/Bl6 mice, long‐term voluntary wheel running is anxiogenic, and focal hippocampal irradiation prevents the increase in anxiety‐like behaviors and neurobiological changes in the hippocampus induced by wheel running. Evidence supports a role of hippocampal 5‐HT 1A receptors in anxiety. Therefore, we investigated hippocampal binding and function of 5‐HT 1A receptors in this mouse model of anxiety. Four weeks of voluntary wheel running resulted in hippocampal subregion‐specific changes in 5‐HT 1A receptor binding sites and function, as measured by autoradiography of [ 3 H] 8‐hydroxy‐2‐(di‐n‐propylamino)tetralin binding and agonist‐stimulated binding of [ 35 S]GTPγS to G proteins, respectively. In the dorsal CA1 region, 5‐HT 1A receptor binding and function were not altered by wheel running or irradiation. In the dorsal dentate gyrus and CA2/3 region, 5‐HT 1A receptor function was decreased by not only running but also irradiation. In the ventral pyramidal layer, wheel running resulted in a decrease of 5‐HT 1A receptor function, which was prevented by irradiation. Neither irradiation nor wheel running affected 5‐HT 1A receptors in medial prefrontal cortex or in the dorsal or median raphe nuclei. Our data indicate that downregulation of 5‐HT 1A receptor function in ventral pyramidal layer may play a role in anxiety‐like behavior induced by wheel running. Synapse 67:648–655, 2013. © 2013 Wiley Periodicals, Inc.