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Differential distribution of PDE4B splice variant mRNAs in rat brain and the effects of systemic administration of LPS in their expression
Author(s) -
ReyesIrisarri Elisabet,
PérezTorres Silvia,
Miró Xavier,
Martínez Emili,
Puigdomènech Pere,
Palacios José M.,
Mengod Guadalupe
Publication year - 2008
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.20459
Subject(s) - phosphodiesterase , in situ hybridization , alternative splicing , rna splicing , inflammation , lipopolysaccharide , microglia , microbiology and biotechnology , adenosine , choroid plexus , gene expression , systemic administration , messenger rna , biology , intracellular , pharmacology , gene , neuroscience , immunology , endocrinology , rna , biochemistry , central nervous system , genetics , in vivo , enzyme
Phosphodiesterases (PDE) control intracellular cyclic adenosine monophosphate (cAMP) levels, which appear to play an important role in the regulation of inflammation. PDE4B is especially important in this process. Using in situ hybridization histochemistry we first mapped the expression sites of the four PDE4B splicing forms in rat brain. Using the systemic administration of the bacterial endotoxin lipopolysaccharide (LPS) as an inflammation model in rats, we found an increase in PDEB2 mRNA expression in choroid plexus. The differential expression of PDE4B spliced forms and the differential regulation of PDE4B2 in an inflammatory model further supports an involvement of this splicing variant in the inflammatory response. Synapse 62:74–79, 2008. © 2007 Wiley‐Liss, Inc.