Premium
Subacute H 2 O 2 , but not poly(IC), upregulates dopamine D2 receptors in retinoic acid differentiated SH‐SY5Y neuroblastoma
Author(s) -
Larouche Annie,
Berube Patrick,
Sarret Philippe,
Grig Sylvain
Publication year - 2008
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.20458
Subject(s) - dopaminergic , dopamine , dopamine receptor d2 , striatum , endocrinology , medicine , sh sy5y , neurotransmission , receptor , chemistry , dopamine receptor , retinoic acid , neuroscience , neuroblastoma , biology , biochemistry , cell culture , genetics , gene
Abnormalities of striatal dopaminergic neurotransmission play a significant role in the pathophysiology of central nervous system disorders such as movement disorders, addictions and schizophrenia. The striatum appears to be exposed to intrinsically high levels of oxidative stress (OS). Little is known, however, on the effect of OS on the regulation of the dopamine D2 receptor (DRD2), a key component of striatal dopaminergic neurotransmission. We report here on the effects of H 2 O 2 (a canonical oxidant and non conventional messenger) and polyinosinic‐polycytidylic acid (poly(IC) which elicits schizophrenia‐like behaviors in newborn rodents and disrupts dopaminergic system development), on DRD2 levels in retinoic acid differentiated SH‐SY5Y neuroblastoma. H 2 O 2 elicited a significant increase in DRD2 mRNA and protein levels. Conversely, poly(IC) did not regulate DRD2 levels, although SH‐SY5Y cells were confirmed to express TLR3 receptors. Under our conditions, H 2 O 2 , but not poly(IC), increased NFκB activation (as assessed by p65 nuclear translocation), which paralleled their effects on DRD2 levels regulation. Synapse 62:70–73, 2008. © 2007 Wiley‐Liss, Inc.