Premium
Effect on [ 11 C]DASB binding after tranylcypromine‐induced increase in serotonin concentration: Positron emission tomography studies in monkeys and rats
Author(s) -
Lundquist Pinelopi,
Roman Magnus,
Syvänen Stina,
Hartvig Per,
Blomquist Gunnar,
HammarlundUdenaes Margareta,
Långström Bengt
Publication year - 2007
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.20382
Subject(s) - tranylcypromine , serotonin transporter , serotonin , positron emission tomography , chemistry , binding potential , monoamine oxidase , 5 ht receptor , endocrinology , medicine , nuclear medicine , biochemistry , receptor , enzyme
Several research groups have demonstrated that under specific conditions, in vivo neuroreceptor binding techniques can be used to measure acute changes in the concentrations of endogenous transmitters in the vicinity of neuroreceptors. The aim of this study was to investigate whether [ 11 C]‐3‐amino‐4‐(2‐dimethylaminomethyl‐phenylsulfanyl)‐benzonitrile ([ 11 C]DASB) binding to the plasma membrane serotonin transporter (SERT) in the rhesus monkey and rat brain decreased after a pharmacologically‐induced increase in the interstitial serotonin (5HT) concentration. Three rhesus monkeys were given repeated single boluses of [ 11 C]DASB in sequential positron emission tomography (PET) experiments. Rats were given the tracer as a bolus dose plus a constant infusion. In vivo binding in both models was studied before and after presumably having increased interstitial 5HT concentrations using tranylcypromine (TCP), which inhibits the enzyme (monoamine oxidase, MAO), that degrades 5HT. The rat brain tissue was analyzed using high‐performance liquid chromatography (HPLC) to determine the proportion of the PET signal comprising unchanged [ 11 C]DASB. The binding of [ 11 C]DASB in the thalamus decreased in both rhesus monkeys and rats after TCP administration. The possibility of using [ 11 C]DASB as a tool for monitoring changes in endogenous serotonin concentrations merits further investigation. Synapse 61:440‐449, 2007. © 2007 Wiley‐Liss, Inc.