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Reverse optical probing (ROPING) of neocortical circuits
Author(s) -
Aaron Gloster,
Yuste Rafael
Publication year - 2006
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.20316
Subject(s) - neuroscience , calcium imaging , neocortex , synapse , neuron , biological neural network , neuronal circuits , computer science , intracellular , biology , calcium , chemistry , organic chemistry , microbiology and biotechnology
We describe an optical technique to detect circuits of synaptically connected neurons. By combining calcium imaging of the spontaneous activity of neuronal populations with intracellular recordings from a given neuron, we perform a type of reverse correlation analysis to detect neurons that generate action potentials time‐locked to the synaptic currents of the recorded cell. This technique can quickly reveal monosynaptically connected neurons. Synapse 60:437–440, 2006. © 2006 Wiley‐Liss, Inc.