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No role of the dopamine transporter in acute ethanol effects on striatal dopamine dynamics
Author(s) -
Mathews Tiffany A.,
John Carrie E.,
Lapa Gennady B.,
Budygin Evgeny A.,
Jones Sara R.
Publication year - 2006
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.20301
Subject(s) - microdialysis , dopamine transporter , dopamine , chemistry , putamen , ethanol , medicine , endocrinology , pharmacology , nomifensine , biochemistry , biology , dopaminergic
The acute effects of ethanol on dopamine (DA) release and clearance in the caudate–putamen were evaluated in wild‐type and dopamine transporter (DAT) knockout (DAT‐KO) mice, using microdialysis and voltammetry. Dialysate DA levels were elevated, ∼80% above baseline levels, after administration of 2 g/kg ethanol in both wild‐type and DAT‐KO mice. In brain slices containing the caudate–putamen, a low (20 mM) concentration of ethanol produced no change in electrically stimulated DA release in either wild‐type or DAT‐KO mice. A high concentration (200 mM) of ethanol caused a similar decrease in DA release in slices from both types of mice. DA clearance was unaltered across the genotypes at low and high concentrations of ethanol. The fact that ethanol had similar effects in wild‐type and DAT‐KO mice, measured by in vivo microdialysis or brain slice voltammetry, supports the idea that acute ethanol does not interact with the DAT to produce its effects on the DA system. Synapse 60:288–294, 2006. © 2006 Wiley‐Liss, Inc.