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Regional expression of 5‐HT 1B receptor mRNA in the human brain
Author(s) -
Varnäs Katarina,
Hurd Yasmin L.,
Hall Håkan
Publication year - 2005
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.20128
Subject(s) - human brain , 5 ht receptor , messenger rna , neuroscience , receptor , expression (computer science) , computational biology , pharmacology , microbiology and biotechnology , biology , medicine , serotonin , computer science , genetics , gene , programming language
The regional mRNA expression pattern of 5‐HT 1B receptors has been extensively characterized in the rodent and guinea pig brain, but a detailed mapping of the 5‐HT 1B receptor mRNA expression in the human brain has not previously been performed. In the present study, the mRNA expression of 5‐HT 1B receptors was analyzed using in situ hybridization histochemistry and whole hemisphere sections of the human postmortem brain. The mRNA expression was compared with the autoradiographic distribution of 5‐HT 1B receptors. High levels of mRNA expression were found in the striatum, cortex, lateral geniculate nucleus, and raphe nuclei. The expression was higher in ventral than in dorsal striatal regions and was absent from the substantia nigra and pallidum, where high levels of 5‐HT 1B receptors were found. A layer‐specific expression pattern was observed in cortical regions. The results extend previous knowledge about the localization of the 5‐HT 1B receptor in the human brain. This study provides evidence of a mismatch of the regional expression of 5‐HT 1B receptor mRNA and the 5‐HT 1B receptor distribution in human brain, similar to what has been demonstrated in other species. This is in line with the localization of this receptor subtype in nerve terminals. The results give support to species differences in the cortical mRNA expression pattern of this receptor subtype. Synapse 56:21–28, 2005. © 2005 Wiley‐Liss, Inc.