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Desensitization and resensitization are independently regulated in human recombinant GluR subunit coassemblies
Author(s) -
Schlesinger Friedrich,
Tammena Derk,
Krampfl Klaus,
Bufler Johannes
Publication year - 2005
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.20110
Subject(s) - homomeric , protein subunit , ampa receptor , receptor , glutamate receptor , microbiology and biotechnology , splice , desensitization (medicine) , biology , silent synapse , chemistry , neuroscience , biochemistry , gene
AMPA‐type glutamate receptor (GluR) channels are the most abundant excitatory transmitter receptors of the central nervous system. Four subunits with different posttranscriptional modifications and flip/flop splice variants are known. In vivo they occur as tetrameric heteromeric receptors. In the present study we analyzed the time course of desensitization (τ D ) and resensitization (τ rec ) kinetics of different homomeric (coassembly of splice or editing variants of one subunit) and heteromeric (coassembly of different subunits) GluR channels. We found that τ D had intermediate values depending on the amount of cDNA of the respective subunit at all heteromeric and homomeric GluR channels tested. The same holds true for τ rec except GluR2 flip channels were coexpressed with GluR1 channels. In this case, τ rec had values close to that of fast resensitizing GluR2 flip channels, even in the case of an abundance of GluR1 cDNA. Synapse 55:176–182, 2005. © 2005 Wiley‐Liss, Inc.