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Sexual experience alters D 1 receptor–mediated cyclic AMP production in the nucleus accumbens of female Syrian hamsters
Author(s) -
Bradley Katherine C.,
Mullins Amanda J.,
Meisel Robert L.,
Watts Val J.
Publication year - 2004
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.20030
Subject(s) - nucleus accumbens , dopamine , caudate nucleus , stimulation , endocrinology , medicine , chemistry , adenylate kinase , cyclase , dopamine receptor d1 , dopamine receptor , neuroscience , receptor , biology
Drugs of abuse produce long‐term changes in dopamine neurotransmission and receptor‐effected intracellular signaling. Similar changes in neuronal activity are mediated by motivated behaviors. To explore cellular mechanisms underlying these neuroadaptations following sexual experience, cyclic AMP accumulation following stimulation of D 1 dopamine receptors, G‐proteins, and adenylate cyclase was compared in the nucleus accumbens and caudate nucleus of sexually naive and experienced female hamsters following sexual behavior. Direct stimulation of adenylate cyclase with forskolin or indirectly by activation of G‐proteins with Gpp(NH)p produced dose‐dependent increases in the formation of cyclic AMP in the nucleus accumbens and caudate nucleus, with no effects of sexual experience on these measures. Specific D 1 receptor stimulation increased Gpp(NH)p‐induced adenylate cyclase activity in the nucleus accumbens and caudate nucleus of all animals. Interestingly, this stimulation was further enhanced only in membranes from the nucleus accumbens, but not from the caudate nucleus, of sexually experienced hamsters compared to the response of naive females. These results demonstrate that sexual behavior experience can sensitize mesolimbic dopamine pathways and that this sensitization occurs through an increase in D 1 receptor–mediated signaling. Synapse 53:20–27, 2004. © 2004 Wiley‐Liss, Inc.