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Influence of restraint stress on the expression and the serine/threonine phosphatase activity of calcineurin in the rat brain
Author(s) -
Takahashi Jun,
Tanaka Kazuhide,
Morinobu Shigeru,
Fujimaki Koichiro,
Li ShengTian,
Kato Kunio,
Ohkawa Masako,
Yamawaki Shigeto,
Kato Nobumasa
Publication year - 2001
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.1034
Subject(s) - dentate gyrus , calcineurin , serine , phosphatase , messenger rna , hippocampus , threonine , endocrinology , in situ hybridization , medicine , northern blot , okadaic acid , biology , chemistry , neuroscience , microbiology and biotechnology , biochemistry , phosphorylation , gene , transplantation
To investigate the role of calcineurin (CaN) in the pathogenesis of stress‐related psychiatric disorders, we examined the expression of CaN A mRNA and the serine/threonine phosphatase activity of CaN in the rat brain following various restraint stress paradigms. Northern blot analysis revealed no significant changes in the levels of CaN A mRNA expression, in either the frontal cortex or the hippocampus, in response to a single restraint stress for either 15, 45, or 90 min. In addition, no significant change in the levels of CaN A mRNA was found 3 or 6 h after a single restraint stress for 90 min. In situ hybridization analysis revealed that a single restraint stress for 45 min had no influence on CaN A mRNA expression in the CA1 and CA3 pyramidal cell layers or in the dentate gyrus granule cell layer of the hippocampus. However, serine/threonine phosphatase activity was significantly increased in both regions in response to a single restraint stress for either 45 or 90 min. These results demonstrate that the single restraint stress paradigms used in this study increase the activity of CaN without any changes in the expression of CaN, suggesting that the activation of calmodulin and the increased levels of heat shock proteins in response to the restraint stress may upregulate the activity of CaN in the rat brain. Synapse 40:130–136, 2001. © 2001 Wiley‐Liss, Inc.