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Detailed distribution of neuropeptide FF receptors (NPFF 1 and NPFF 2 ) in the rat, mouse, octodon, rabbit, guinea pig, and marmoset monkey brains: A comparative autoradiographic study
Author(s) -
Gouardères Christine,
Puget Alain,
Zajac JeanMarie
Publication year - 2003
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.10305
Subject(s) - receptor , hypothalamus , guinea pig , biology , marmoset , medicine , endocrinology , neuropeptide , central nervous system , biochemistry , paleontology
The distribution of neuropeptide FF receptors (NPFF 1 and NPFF 2 ) was analyzed throughout the central nervous system of rodents (rat, mouse, Octodon degus, and guinea pig), rabbit, and marmoset monkey brains, representing three orders of mammals. Quantitative in vitro receptor autoradiography with [ 125 I]EYF ([ 125 I]EYWSLAAPQRF‐NH 2 ) and [ 125 I]YVP ([ 125 I]YVPNLPQRF‐NH 2 ) as specific radioligands for NPFF 2 and NPFF 1 receptors, respectively, was used. The NPFF 2 receptor is predominantly expressed in all species, except in the central nervous system of Octodon degus , in which it is undetectable. The density of the NPFF 1 subtype is low in rat and mice, moderate in octodon, rabbit, and monkey, and relatively high in the guinea pig. The present study reveals prominent species differences in the NPFF receptors expression in the brain. The distribution pattern of NPFF 2 receptors in the diencephalon and the superficial layers of the spinal cord is consistent with a hypothesized potential role for NPFF in the modulation of sensory input and opioid analgesia. In contrast, the constant presence of NPFF 1 receptors in the septum, the nucleus of the tractus solitarius, and the hypothalamus suggest its participation in neuroendocrine functions. Synapse 51:249–269, 2004. © 2003 Wiley‐Liss, Inc.