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In vivo assessment of adenoviral vector‐mediated gene expression of dopamine D 2 receptors in the rat striatum by positron emission tomography
Author(s) -
Umegaki Hiroyuki,
Ishiwata Kiichi,
Ogawa Osamu,
Ingram Donald K.,
Roth George S.,
Yoshimura Juri,
Oda Keiichi,
MatsuiHirai Hisako,
Ikari Hiroyuki,
Iguchi Akihisa,
Senda Michio
Publication year - 2001
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/syn.10035
Subject(s) - raclopride , in vivo , positron emission tomography , striatum , radioligand , ex vivo , binding potential , dopamine , neuroscience , biology , microbiology and biotechnology , chemistry
For functional assessment of gene therapy in experimental animals, in vivo assessment of transferred genes will provide a major advance over an in vitro analysis which must be done post‐hoc. In the current study we conducted positron emission tomography (PET) analysis in rats following injection of the adenoviral vector encoding the cDNA for the rat dopamine D 2 receptors (D 2 R) (AdCMV.DopD 2 R) into rat brain to provide a quantitative evaluation of D 2 R overexpression. Quantitative measurements as well as images by PET and ex vivo autoradiography demonstrated the significant increase of D 2 R binding of [ 11 C]raclopride, a specific D 2 R radioligand, in the AdCMV.DopD 2 R‐injected rat striatum 2 or 3 days after vector injection. Longitudinal in vivo assessment of the gene expression by PET demonstrated decreased binding of [ 11 C]raclopride with time, which was in agreement with the observation in a cross‐sectional autoradiographic study. The results of the current study demonstrate that PET can be used for longitudinal in vivo assessment of D 2 R expression mediated by adenoviral vector in rat brain. Synapse 43:195–200, 2002. © 2002 Wiley‐Liss, Inc.