Open AccessPhenotypic and functional characterization of the hematopoietic stem cell
Author(s) -
Sharkis Saul J.,
Collector Michael I.,
Barber James P.,
Vala Milada S.,
Jones Richard J.
Publication year - 1997
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.5530150807
Subject(s) - biology , stem cell , haematopoiesis , phenotype , hematopoietic stem cell , bone marrow , stem cell theory of aging , microbiology and biotechnology , cell , immunology , stem cell factor , genetics , gene
The purpose of this report is to demonstrate the phenotypic and functional characteristics of a primitive hematopoietic stem cell (HSC). We present evidence that an isolated murine HSC can repopulate the hematopoietic tissues of lethally irradiated recipient animals long term. By limiting dilution, as few as ten isolated stem cells can reconstitute mice for their lifetime. The stem cell which we have isolated does not copurify with colony forming unit‐spleen or radioprotect recipients from lethal radiation. The phenotypic characterization of this rare cell, which represents 0.005% of total bone marrow, includes either the absence or very low expression of markers associated with long‐term repopulating cells described by other groups. We believe this stem cell represents a very early self‐renewing stem cell in the mouse. Stem Cells 1997; 15(suppl 1): 41–45