Open AccessPromotion of survival and proliferation by interleukin 3, fcil‐ligand and erythropoietin on early and late appearing spleen colony forming units in culture
Author(s) -
Sasaki Hideki,
Ikuta KoIchiro,
Matsuyama Shusuke,
Hirabayashi Yoko,
Inoue Tohru,
Taniguchi Satoshi
Publication year - 1993
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.5530110625
Subject(s) - erythropoietin , biology , colony forming unit , spleen , stem cell factor , andrology , cfu gm , growth factor , immunology , microbiology and biotechnology , stem cell , progenitor cell , receptor , endocrinology , biochemistry , genetics , medicine , bacteria
We examined the effects of interleukin 3 (IL‐3), kit ligand and erythropoietin (EPO) on the survival and growth of early appearing spleen colony forming units (CFU‐S 8 ) and late appearing CFU‐S (CFU‐S 12 ) in short‐term liquid culture (SLC). In the control cultures, without any additive, CFU‐S 8 and CFU‐S, 2 declined; nearly 10% of the initial number of CFU‐S still survived by the second day of culture. The addition of IL‐3 or kit .‐ligand increased the survival both of CFU‐S, and CFU‐S 12, with an increased dose of concentration, at final concentrations of 10‐200 U/ml and 500‐50 dilution, respectively. However, only the survival of CFU‐S, increased when EPO was added up to 10 U/ml, while the frequency of CFU‐S 12 was not higher than in the control culture. The percentage of CFU‐S 8 and CFU‐S 12 in DNA synthesis, evaluated in 3 H‐TdR cytocide experiments, increased after one day in culture with each of the three factors. The results suggest that all three factors stimulated the proliferation of both populations of CFU‐S, but the two populations showed different patterns of response to each factor; EPO stimulates the proliferation of CFU‐S 12 that differentiate into CFU‐S 8 that have less capacity for self‐renewal, while the addition of IL‐3 or kit‐ligand causes an increase in the number of both populations due to the stimulation of an earlier stage of stem cell differentiation or self‐renewal of CFU‐S 12 . Our experimental system (SLC‐CFU‐S assay) is useful for evaluating the response of hematopoietic stem cells to cytokines which promote the in vitro survival and proliferation of these cells.