Open AccessA Phenotypic Switch of Differentiated Glial Cells to Dedifferentiated Cells Is Regulated by Folate Receptor α
Author(s) -
Monick Sarah,
Mohanty Vineet,
Khan Mariam,
Yerneni Gowtham,
Kumar Raj,
Cantu Jorge,
Ichi Shunsuke,
Xi Guifa,
Singh Bal Ram,
Tomita Tadanori,
Mayanil Chandra Shekhar
Publication year - 2019
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.3067
Subject(s) - biology , sox2 , klf4 , microbiology and biotechnology , cellular differentiation , phenotype , transcription factor , gene knockdown , stem cell , small hairpin rna , cell culture , genetics , gene
In a previous study, we showed that folate receptor‐α (FRα) translocates to the nucleus where it acts as a transcription factor and upregulates Hes1 , Oct4 , Sox2 , and Klf4 genes responsible for pluripotency. Here, we show that acetylation and phosphorylation of FRα favor its nuclear translocation in the presence of folate and can cause a phenotypic switch from differentiated glial cells to dedifferentiated cells. shRNA‐FRα mediated knockdown of FRα was used to confirm the role of FRα in dedifferentiation. Ocimum sanctum hydrophilic fraction‐1 treatment not only blocks the folate mediated dedifferentiation of glial cells but also promotes redifferentiation of dedifferentiated glial cells, possibly by reducing the nuclear translocation of ~38 kDa FRα and subsequent interaction with chromatin assembly factor‐1. Stem Cells 2019;37:1441–1454