Open AccessAdult DRG Stem/Progenitor Cells Generate Pericytes in the Presence of Central Nervous System (CNS) Developmental Cues, and Schwann Cells in Response to CNS Demyelination
Author(s) -
Vidal Marie,
Maniglier Madlyne,
Deboux Cyrille,
Bachelin Corinne,
Zujovic Violetta,
BaronVan Evercooren Anne
Publication year - 2015
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.1997
Subject(s) - biology , neurosphere , stem cell , progenitor cell , neural stem cell , neuroscience , adult stem cell , neural crest , transplantation , microbiology and biotechnology , cellular differentiation , embryo , medicine , biochemistry , gene
It has been proposed that the adult dorsal root ganglia (DRG) harbor neural stem/progenitor cells (NPCs) derived from the neural crest. However, the thorough characterization of their stemness and differentiation plasticity was not addressed. In this study, we investigated adult DRG‐NPC stem cell properties overtime, and their fate when ectopically grafted in the central nervous system. We compared them in vitro and in vivo to the well‐characterized adult spinal cord‐NPCs derived from the same donors. Using micro‐dissection and neurosphere cultures, we demonstrate that adult DRG‐NPCs have quasi unlimited self‐expansion capacities without compromising their tissue specific molecular signature. Moreover, they differentiate into multiple peripheral lineages in vitro. After transplantation, adult DRG‐NPCs generate pericytes in the developing forebrain but remyelinating Schwann cells in response to spinal cord demyelination. In addition, we show that axonal and endothelial/astrocytic factors as well astrocytes regulate the fate of adult DRG‐NPCs in culture. Although the adult DRG‐NPC multipotency is restricted to the neural crest lineage, their dual responsiveness to developmental and lesion cues highlights their impressive adaptive and repair potentials making them valuable targets for regenerative medicine. S tem C ells 2015;33:2011–2024