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Conformational Analysis of Carboxymethylamylose by Potentiometric Titration and SAXS
Author(s) -
Takada Masako,
Wada Kaori,
Muroga Yoshio,
Ikake Hiroki,
Shimizu Shigeru
Publication year - 2018
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.201800016
Subject(s) - potentiometric titration , aqueous solution , amylose , chemistry , titration , small angle x ray scattering , crystallography , titration curve , solubility , conformational change , circular dichroism , ion , stereochemistry , inorganic chemistry , organic chemistry , scattering , starch , physics , optics
Hydroxyl groups of amylose are barely dissociated into ions at pH lower than about 12, and so amylose is almost insoluble in neutral and acidic aqueous solutions and its conformation in solution has been a question under debate. Carboxymethylamylose (CMA) has much better solubility in aqueous solution than amylose and the conformation of CMA is expected to reflect that of the parent amylose. In the present study, CMA was prepared by substituting H of 24.5 mol.% hydroxyl groups of amylose with carboxymethyl groups, and the conformation of CMA in KI aqueous solution was studied as a function of the concentration of iodine I 2 and the degree of ionization α by potentiometric titration. Different conformational behaviors were observed in different α domains: In a low‐α domain, CMA in KI/I 2 aqueous solution and in KI aqueous solution behaves as a tightly‐wound helical chain and a loosely‐wound helical chain, respectively. The helical‐ and psedo‐helical‐chain are gradually collapsed to extended‐coils as α increases and CMA equally behaves as an extended‐coil in a medium‐α domain and as a more extended‐coil in a higher‐α domain. Such a conformational change was reflected in a change of persistence length of a chain with α, which was clarified by small‐angle X‐ray scattering study.