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Immobilization of α ‐amylase on reactive modified fiber and its application for continuous starch hydrolysis in a packed bed bioreactor
Author(s) -
Temoçin Zülfikar
Publication year - 2014
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.201300132
Subject(s) - hydrolysis , amylase , starch , chemistry , immobilized enzyme , bioreactor , chromatography , michaelis–menten kinetics , packed bed , fiber , enzyme , enzyme assay , biochemistry , organic chemistry
In this study, the enzyme α ‐amylase was immobilized on reactive modified poly (ethylene terephthalate) fiber. The activities of free α ‐amylase and immobilized α ‐amylase were compared in a batch starch hydrolysis system. Optimum pH and Michaelis–Menten constants were determined for both free α ‐amylase and immobilized α ‐amylase. The immobilization shifted the optimum pH to a higher level. The Michaelis–Menten constant values ( K m ) of the immobilized α ‐amylase and free α ‐amylase were obtained as 11.94 and 6.61 mg/mL, respectively. The experiments indicated that the immobilization increased the thermal stability of the α ‐amylase. The immobilized α ‐amylase, easily separated from the reaction media, could be used effectively for 15 cycles with 65% retention of the initial activity. Moreover, the immobilized α ‐amylase was effectively used in packed bed bioreactor for continuous starch hydrolysis throughout 6.5 h without loss of activity. Different starches ( e.g ., potato and wheat) were hydrolyzed by the immobilized α ‐amylase in continuous process. The results indicate a potential use of this immobilized enzyme system for the construction of packed bed bioreactors to be used in the hydrolysis of the starch.