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Physical properties and structure of enzymatically synthesized amylopectin analogs
Author(s) -
Ciric Jelena,
Woortman Albert J. J.,
Gordiichuk Pavlo,
Stuart Marc C. A.,
Loos Katja
Publication year - 2013
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.201300063
Subject(s) - amylopectin , branching (polymer chemistry) , polymerization , chemistry , degree of polymerization , glycogen phosphorylase , dynamic light scattering , polysaccharide , size exclusion chromatography , glycogen branching enzyme , enzyme , chromatography , polymer , organic chemistry , materials science , starch , nanotechnology , nanoparticle , amylose
The mechanism of the enzymatic polymerization of amylopectin analogs with phosphorylase b and glycogen branching enzyme is very intriguing. Recently, size exclusion chromatography with multi‐detection of enzymatically synthesized amylopectin analogs in combination with MALDI‐ToF MS analysis of enzymatically debranched analogs was used to solve parts of the molecular mechanism of analog's enzymatic polymerization. In this work dynamic light scattering (DLS), AFM and cryo‐TEM, respectively were used to determine structural characteristics of the same analogs. The results were compared with SEC analyses. The presented analyses in this work fully agreed with the recently made observations and confirmed the changes in the architecture of the synthesized polysaccharide due to the change of enzymatic polymerization mechanism. Furthermore, we showed that the synthetic amylopectin analogs are stable to retrogradation at 4°C if the main side chain length is no longer than 12 glucose units and that they have mostly fluid‐like behavior in the form of 20% suspensions.