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Molecular characteristics of native sago starch and isolated fractions determined using asymmetrical flow field‐flow fractionation
Author(s) -
Juna Shazia,
Huber Anton
Publication year - 2012
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.201100100
Subject(s) - starch , amylose , amylopectin , fractionation , analytical chemistry (journal) , field flow fractionation , molar mass , chemistry , chromatography , mass fraction , melt flow index , fraction (chemistry) , materials science , organic chemistry , polymer , copolymer
The apparent average molar masses ( M w,app ), apparent average radii of gyration ( R g,app ), of native sago starch and fractions were determined using asymmetrical flow field‐flow fractionation coupled with multi‐angle light scattering and refractive index detectors (AF4/MALS/RI). Amylose‐type (Fraction A) and amylopectin‐type (Fraction B) were chemically separated from native sago starch. Native sago starch and Fractions (A–B) were dissolved in 1M KSCN using a high pressure microwave vessel. The effect of varying cross flow rates at a fixed channel flow rate upon the M w,app and R g,app distributions of native sago starch and Fractions (A–B) were investigated. The average M w,app values of native sago starch, Fraction (A) and Fraction (B) were 60 × 10 6 , 1.5 × 10 6 and 60 × 10 6 g/mol, respectively, with average R g,app values of 142, 75 and 127 nm, respectively. The sphere‐equivalent hydrodynamic radii ( R h ) values for native sago starch and fractions were determined from AF4 experimental parameters.