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High‐Speed Separation and Size Characterization of Wheat and Barley Starch Granules by Lift‐Hyperlayer Asymmetrical Flow Field‐Flow Fractionation in Synergy with SPLITT Fractionation
Author(s) -
Contado Catia,
Wahlund KarlGustav
Publication year - 2006
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.200300198
Subject(s) - fractionation , field flow fractionation , starch , chemistry , characterization (materials science) , chromatography , flow (mathematics) , lift (data mining) , continuous flow , materials science , analytical chemistry (journal) , nanotechnology , mechanics , computer science , food science , physics , data mining
The complementary employment of two separation techniques, one analytical‐scale, the other preparative‐scale, i.e. the lift‐hyperlayer asymmetrical flow field‐flow fractionation (AsyFlFFF) and the split flow‐thin cell (SPLITT) is presented for the first time as a methodology to size‐separate and rapidly characterize starch granules of different botanical origins. Very rapid separation and size characterization is achieved by AsyFlFFF and the techniques are mutually used to characterize and confirm data produced by the other. Light microscopy (LM) was used to test the quality of the performed SPLITT separations and consequently to evaluate the AsyFlFFF results. The procedure was applied to wheat and barley starch.