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Kinetics and Modelling of the Enzyme Hydrolysis of Maltose with Free and Immobilized Glucoamylase
Author(s) -
Yankov Dragomir,
Beschkov V.,
Rouleau Denis
Publication year - 1997
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19970490708
Subject(s) - michaelis–menten kinetics , maltose , chemistry , kinetics , hydrolysis , immobilized enzyme , enzyme kinetics , diffusion , thermodynamics , reaction rate constant , chromatography , product inhibition , enzyme , enzyme assay , organic chemistry , non competitive inhibition , active site , physics , quantum mechanics
The kinetics of the maltose hydrolysis by means of free and immobilized in polyacrylamide gel glucoamylase has been investigated. The kinetic constants in the Michaelis‐Menten's equation have been determined. In the case of immobilized enzyme two different methods have been used, the one giving intrinsic constant values and the other – affected by the diffusion resistances. The obtained kinetic parameters were compared with these determined for free enzyme system. A mathematical model of the process has been constructed. The inhibition effect of the product and the reversibility of the reaction have been taken into account. The model has been solved using already determined kinetics parameters. Very good correlation between calculated and experimental data was found.